Our team brings together expertise that spans biomedical, biophysical, chemical, computational, instrumentation and engineering disciplines.
We have developed innovative flow cytometric tools for discovery research that enable homogeneous analysis of ligand binding and protein-protein interaction, HT sample handling, high content analysis, and real-time measurements of cell response. We have already achieved delivery rates of µl sized samples from multiwell plates at rates up to 100 samples/min end point assays and multiplex rates up to 1000/min. Yielding projected capacity: 5,000-10,000 wells per day per detector. We have completed screens on several small molecule libraries, discovering novel small molecules that bind to a GPCR peptide receptor.
Our experience indicates that virtually any molecular assembly or cell response can be displayed in a format compatible with flow cytometry. Moreover, by creating a suspension array of particles, assays and responses can be highly multiplexed or performed on complex cell populations without loss of throughput. Our novel sampling approach (HyperCyt®) makes flow cytometry an attractive platform for drug discovery, proteomics, and real-time analysis of molecular interactions. Flow cytometry is particularly convenient for alternately assessing both cellular and molecular activities of small molecules.
- G protein-coupled receptors;
- Integrins and adhesion molecules;
- Cell cycle;
- Protein-protein interactions;
- Protein-nucleic acid interactions;
- Kinase assays
- State-of-the-art, innovative tools for ligand-based and structure-based
- Unique tools for post-HTS analyses, clustering and
in silico profiling;
- High performance tools for bioinformatics
- Unique data archiving and data mining capabilities;
- Tools to
evaluate post-HTS analyses